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mouse leydig tumor cells mltcs  (ATCC)
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ATCC mouse leydig tumor cells mltcs
Figure 1. Plasma membranes can support steroidogenesis. PMs from rat adrenals, <t>MLTCs,</t> Y1 adrenocortical cells, and liver were purified and added to the in vitro mitochondrial reconstitution assay, in the absence of any other sources of cholesterol or accessory proteins. Pregnenolone production was measured by ELISA. A) The relative pregnenolone production was measured with rat adrenal PMs, MLTC, Y1 adrenocortical cell, and liver PMs as the sources of cholesterol. The basal pregnenolone production for mitochondria without PMs ranged from 0.5 to 3 ng/ml. B) Comparison of rat adrenal PMs and lipid emulsion as cholesterol sources for pregnenolone production in the absence or presence of accessory proteins. LE, lipid emulsion; Mito, mitochondria; NS, not significant; SNAREs/StAR, a cocktail containing recombinant SNAP23, SNAP25, a-SNAP, STX17, and N62-StAR. Results are representative of 3 independent experiments (n = 3). **P , 0.01, ***P , 0.001.
Mouse Leydig Tumor Cells Mltcs, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. Plasma membranes can support steroidogenesis. PMs from rat adrenals, MLTCs, Y1 adrenocortical cells, and liver were purified and added to the in vitro mitochondrial reconstitution assay, in the absence of any other sources of cholesterol or accessory proteins. Pregnenolone production was measured by ELISA. A) The relative pregnenolone production was measured with rat adrenal PMs, MLTC, Y1 adrenocortical cell, and liver PMs as the sources of cholesterol. The basal pregnenolone production for mitochondria without PMs ranged from 0.5 to 3 ng/ml. B) Comparison of rat adrenal PMs and lipid emulsion as cholesterol sources for pregnenolone production in the absence or presence of accessory proteins. LE, lipid emulsion; Mito, mitochondria; NS, not significant; SNAREs/StAR, a cocktail containing recombinant SNAP23, SNAP25, a-SNAP, STX17, and N62-StAR. Results are representative of 3 independent experiments (n = 3). **P , 0.01, ***P , 0.001.

Journal: The FASEB Journal

Article Title: Plasma membrane cholesterol trafficking in steroidogenesis

doi: 10.1096/fj.201800697rrr

Figure Lengend Snippet: Figure 1. Plasma membranes can support steroidogenesis. PMs from rat adrenals, MLTCs, Y1 adrenocortical cells, and liver were purified and added to the in vitro mitochondrial reconstitution assay, in the absence of any other sources of cholesterol or accessory proteins. Pregnenolone production was measured by ELISA. A) The relative pregnenolone production was measured with rat adrenal PMs, MLTC, Y1 adrenocortical cell, and liver PMs as the sources of cholesterol. The basal pregnenolone production for mitochondria without PMs ranged from 0.5 to 3 ng/ml. B) Comparison of rat adrenal PMs and lipid emulsion as cholesterol sources for pregnenolone production in the absence or presence of accessory proteins. LE, lipid emulsion; Mito, mitochondria; NS, not significant; SNAREs/StAR, a cocktail containing recombinant SNAP23, SNAP25, a-SNAP, STX17, and N62-StAR. Results are representative of 3 independent experiments (n = 3). **P , 0.01, ***P , 0.001.

Article Snippet: Mouse Leydig tumor cells (MLTCs) were obtained from ATCC and were cultured in RPMI 1640 plus 10% fetal bovine serum, 100 U/ml penicillin, and 100 g/ml streptomycin.

Techniques: Clinical Proteomics, In Vitro, Reconstitution Assay, Enzyme-linked Immunosorbent Assay, Comparison, Emulsion, Recombinant